sean_dublin said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
HPLC results interpretation for certificates of analysis: when you get your Janoshik report back, here's what the numbers mean:
- Purity %: Should be >97%. Below 95% is concerning.
- Retention time: Should match reference standard for the peptide
- Related substances: Degradation products should be <2% total
- Single impurity: No individual impurity should exceed 1%
My last batch: 99.7% purity, 0.1% total related substances. Clean chromatogram with single sharp peak at expected retention time. Exactly what you want to see.
paige_pharma said:Agreed, and mass spec answers a question purity does not: identity.
Bookmarking. The distinction being drawn above is the one nobody else makes. Printing the relevant bit and taking it with me.
sean_dublin said:My HPLC chromatogram analysis for certificates of analysis — I sent my batch to Janoshik and here's what the results showed: Main peak retention time:…
COA red flags for certificates of analysis — here's what to look for when reviewing a Certificate of Analysis:
- No HPLC data: If there's no chromatography graph, it's not a real COA
- Exact 100% purity: Suspicious — real testing always shows slight variation
- No batch number: Can't trace back to specific production
- No endotoxin testing: For injectables, this is essential
- Template looks like it was made in Word: Professional labs have standardized formats
I have seen fake COAs that are literally photoshopped. Always verify independently if possible.
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View Resultsnewstart_MO said:I have a certificate reporting 99.2% purity and 6.1mg content on a vial sold as 5mg, and I cannot work out whether those two numbers are consistent or…
Community-verified vendors for certificates of analysis: I maintain a spreadsheet of Janoshik-verified test results submitted by community members. Across 46 submitted tests:
- 82% tested at ≥98% purity
- 12% tested at 95-98% purity
- 6% tested at <95% purity (concerning)
The vendors with consistently high purity results are listed in the Vendor Directory. We update monthly based on new community submissions.
Moderator note: two posts asking for a source have been merged into one. Please search the thread before asking again. No action needed from anybody.